PFKFB2 antibody (AA 336-385)

Catalog No. ABIN6746455

This Rabbit Polyclonal antibody specifically detects PFKFB2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Monkey, Dog, Horse, Zebrafish (Danio rerio) and Chickenand has been mentioned in 1 publication.

Quick Overview for PFKFB2 antibody (AA 336-385) (ABIN6746455)

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Reactivity: Human, Mouse, Rat, Monkey, Dog, Horse, Zebrafish (Danio rerio), Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PFKFB2 Antibody

Binding Specificity: AA 336-385

Specificity: Mouse PFKFB2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Chicken (100%) Bovine, Rabbit, Pig (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa336-385 of mouse Pfkfb2 (Q6GTL7, NP_032851). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus (100%), Bovine, Bat, Rabbit, Pig, Guinea pig, Xenopus (92%), Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PFKFB2 antibody (ABIN6746455)

Jaiswal, Marlow, Gupta, Narayan: "Beta-catenin-mediated transactivation and cell-cell adhesion pathways are important in curcumin (diferuylmethane)-induced growth arrest and apoptosis in colon cancer cells." in: Oncogene, Vol. 21, Issue 55, pp. 8414-27, (2002) (PubMed).

Target Details for PFKFB2

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Alternative Name: PFKFB2

Background: Name/Gene ID: PFKFB2

Synonyms: PFKFB2, 6PF-2-K/Fru-2,6-P2ase 2, PFK-2/FBPase-2, PFK/FBPase 2, PFKFB, cardiac

Gene ID: 5208

NCBI Accession: NP_032851

UniProt: O60825

Pathways: PI3K-Akt Signaling, Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process